Table 3 Antibodies and procedures for fluorescent immunostaining

Antigen Clone/Sera Supplier Dilution Secondary procedurea

Cytokeratin 18 (FITC conjugate) CY90 Sigma 1:20
Cytokeratins 1, 5, 10, 14 34ßE12 Dako 1:50 RB, SF
Vimentin VIM 13.2 Sigma 1:200 RF
PSA Rabbit Dako 1:20 SB, SF
PSMA PSM-P12 JM Villete 1:100 RF
AR NCL-AR-318 Novocastra 1:50 RB, SF
Desmoglein CBL 174 Cymbus Biotechnology 1:10 RB, SF
E-Cadherin HECD-1 R&D 10 µg/ml RB, SF
ß1-Integrin sc-9970 Santa Cruz 2 µg/ml RF
CD44 F10 44-2 Novocastra 1:10 RB, SF

a RF, 1:30 dilution of FITC conjugated rabbit antimouse; RB, 1:300 dilution of biotinylated rabbit antimouse; SF, 1:50 dilution of FITC-conjugated streptavidin; SB, 1:300 dilution of biotinylated swine antirabbit.