Fig. 9. Examples of immunohistochemical staining of prostatic epithelial Matrigel spheroids. All spheroids shown were grown in KSFM, except for that illustrating AR expression, which was cultured in the presence of KSFM plus 2% serum, 20-7 M dihydrotestosterone, 10 ng/ml estrogen, and primary stroma. Spheroid nuclei were counterstained with DAPI (blue). The epithelial sample C was cultured for 2 weeks. Bar, 80 µm.