Fig. 8. Polarization of PSA and ß1-integrin in Matrigel epithelial spheroids when cocultured with stroma. Using confocal analysis, the expression of PSA and ß1-integrin was compared between epithelial spheroids (sample C) grown in KSFM plus 2% serum (K2) or K2 plus primary stroma (S), 20-7 M dihydrotestosterone (D) and 10 ng/ml estrogen (O) for 2 weeks. Bar, 70 µm.