Fig. 3. Flow cytometric analyses of L-PHA binding, and expression of LAMP-1 and ß1-integrin on the cell surface of high and low metastatic hybrids, parental Cloudman melanoma cells, and normal peritoneal macrophages. A, histogram of fluorescent intensity with (---) or without (----) FITC-conjugated L-PHA (left), rat antimouse LAMP-1 (middle), and rat antimouse CD29 for ß1-integrin (right). Cell lines are indicated on the right. B, quantitation of data from flow cytometric analyses. Fluorescent intensity relative to negative controls, representing mean from five pooled experiments. *P < 0.01; **P < 0.001; bars, ±SD.