Fig. 6. Radicicol does not inhibit p90RSK phosphorylation. The same extracts as those used for Fig. 4 were analyzed by Western blotting for phosphorylation status of p90RSK. Blots were first probed with anti-phospho-p90RSK (top panels) and subsequently stripped and reprobed with anti-p90RSK (bottom panels). A, ras-transformed NIH3T3 cells. B, MEK-1-S218D,S222D-infected NIH3T3 cells.