Fig. 4. T-STAR lacking the COOH-terminal 20 aa is localized in the cytoplasm. A, three different myc-tagged expression constructs were made: T-STAR containing the wt gene; STAR lacking the NH2-terminal 150 aa containing almost the entire STAR domain; and NLS lacking the 20 C00H-terminal aa. *, predicted nuclear localization signal. B, T-STAR and NLS constructs were transfected into VH10/SV postcrisis cells, and cellular localization was determined using an anti-myc antibody. Nuclei were stained using 4',6-diamidino-2-phenylindole.