Fig. 6. STAT5B induces cell motility in response to v-Src activation. Cell migration assays in which cells migrate through a gelatin-coated 10-µm-pore size filter modified Boyden chamber were performed as described in "Materials and Methods." Cell suspensions (5 x 106 cells/500 µl) were added to the top compartment. DMEM/10% BCS was added to the lower compartment. After 12 h, the number of cells that had migrated to the lower compartment was determined.