Fig. 3. STAT5B minimally promotes cell growth in the absence of serum. Cell proliferation assays of stably transfected NIH-3T3 cells were performed using CellTiter 96 AQ (Promega). Cells (see the Fig. 1 legend) were plated in 98-well plates (2 x 103 cells/well) in triplicate for 12 h in complete medium and then seeded for 36 h without serum. The experiment was repeated twice. 1 is the relative value of the absorbance of the cells transfected alone with control vectors. Error bars, SD.