Fig. 3. The effects of Nm23-H1 and Nm23-H1F expression on maturation induced by various concentrations of progesterone. Oocytes were microinjected with water (Control, {circ}) or with Nm23-H1 (•) or Nm23-H1F ({blacktriangleup}) mRNA (50 ng/oocyte) and incubated for 20–24 h. The oocytes were then incubated with (A) 5, (B) 0.5, (C) 0.1, and (D) 0.01 µg/ml progesterone as indicated and scored for GVBD. Data shown are from one of two similar experiments.