Fig. 2. The effects of acidic (Nm23-H1 and Nm23-H1F) and basic (Nm23-H2 and Nm23-H2F) isoforms of Nm23 on progesterone-induced GVBD. Oocytes were microinjected with mRNA (50 ng/oocyte) for Nm23-H1 (A, •), Nm23-H2 (A, {blacktriangleup}), Nm23-H1F (B,•), or Nm23-H2F (B, {blacktriangleup}) or microinjected with water (A and B, Control, {circ}). Oocytes were incubated for 20 or 24 h for full expression Nm23 and then stimulated with 5 µg/ml progesterone. At least 20 microinjected oocytes were followed for each experimental group, and the results were repeated.