Fig. 1. Effects of Nm23-H1 and Nm23-H1F on maturation. A, Nm23 immunoblot showing the endogenous Mr 24,000 Nm23 protein and the ectopic Mr 19,000 human Nm23 proteins. Oocytes were microinjected with water (Lane 1) or synthetic mRNAs for Nm23-H1 (Lanes 24) or Nm23-H1F (Lanes 57). Oocytes were incubated for 2024 h in the absence of progesterone to allow full expression of Nm23 and then incubated for 10 h in the presence of progesterone. BD, time course of maturation in oocytes microinjected with water (Control, ) or mRNAs for Nm23-H1 () or Nm23-H1F (). The amount of RNA injected ranged from 974 ng/oocyte, as indicated. GVBD was scored by the appearance of a white dot at the oocytes animal pole. Ten to 20 oocytes were followed in each experimental group. Data shown are from one of two similar experiments.