Fig. 6. Under reduced serum conditions, JunD levels increase in AKR-2B cells. Subconfluent cultures of murine AKR-2B cells were incubated in growth medium supplemented with 10% (Lanes 1, 3, 5, and 7) or 1% FBS (Lanes 2, 4, 6, and 8) for 1 day (Lanes 1 and 2), 2 days (Lanes 3 and 4), 3 days (Lanes 5 and 6), and 4 days (Lanes 7 and 8). Nuclear extracts were prepared, and extracts containing equal amounts of proteins were analyzed by immunoblotting using the anti-JunD antibody. The same membrane was subsequently probed with antibodies to ß-actin (bottom panel) to control loading of equal amounts of proteins in the lanes. An arrow indicates the JunD protein band.