Fig. 5. An increase in p202 levels under reduced serum conditions correlates with cell growth inhibition. A, subconfluent cultures of AKR-2B cells (in duplicates) were incubated in growth medium supplemented with either 10% ({blacksquare}) or 1% serum ({square}) for 0 day, 2 days, or 4 days. Cells were counted three times in a hemocytometer for each time point, and the average cell number is indicated. B, subconfluent cultures of AKR-2B cells (in duplicates) were incubated in growth medium supplemented with either 10% (left panels) or 1% FBS (right panels) for 2 days (top panels) or 4 days (bottom panels). Harvested cells (5 x 105) were processed for flow cytometry as described in "Materials and Methods." The samples were subjected to flow cytometry (FACStar PLUS; Becton Dickinson) and analyzed using ModFit LT version 2.0 software (Variety Software House). The G1:S-phase ratio is indicated.