Table 1 IFN-{gamma}-mediated growth inhibition is associated with an increase in G0-G1 and a decrease in S phase and G2-M phase of the cell cycle

MCF-7 and MDA-MB-231 cells were cultured in IMEM plus 5% FCS and treated with 10 ng/ml IFN-{gamma} for 24 h. Cells were fixed in ethanol, stained with propidium iodide, and sorted by DNA content. Data shown are the mean of duplicate samples ± SD. Data shown are representative of three separate experiments.

G0-G1 P S phase P G2-M phase P

MCF-7
Control 44.4 ± 3.1 37.7 ± 3.1 17.9 ± 0.2
IFN-{gamma} 53.2 ± 1.0a 0.05 31.9 ± 0.5 0.10 14.5 ± 1.5 0.17
MDA-MB-231
Control 51.2 ± 3.8 33.4 ± 2.3 15.3 ± 1.6
IFN-{gamma} 66.9 ± 1.7a 0.04 25.9 ± 0.4a 0.04 7.1 ± 1.5a 0.03

a P <= 0.05.