Fig. 3. IFN-{gamma} inhibits anchorage-independent growth of MCF-7 cells but not MDA-MB-231 cells. MCF-7 and MDA-MB-231 cells were plated in soft agar with or without 10 ng/ml IFN-{gamma}. Colonies were allowed to form and were counted after 10 days. Error bars, the mean of duplicate samples ± SE.