Fig. 5. Expression of transfected p53 with or without 10 nM PMA. Cells at an initial concentration of 0.2 x 106 cells/ml were incubated with or without PMA at 10 nM at 32°C (i.e., the temperature permissive for the wild-type conformation of p53). After 24 h, the cells were subjected to Western blot. Arrow to the right, position of p53 protein at Mr 53,000.