Fig. 2. Proliferation (A) and viability (B) of mock-transfected and p53-expressing K562 clones. Cells at an initial concentration of 0.2 x 106 cells/ml were grown in suspension culture at 32°C (i.e., the temperature permissive for the wild-type conformation of p53) for 4 days. Viability, as judged by trypan blue exclusion, as well as the total number of cells, was determined daily. Mean values are from four separate experiments. , Mock 1; , Mock 2; , ptsp53/A2; , ptsp53/A4; , ptsp53/A5; , ptsp53/A10.