a MCF-7 and T-47D cells were plated in triplicate in 24-well plates at densities of 1 x 104 and 2.5 x 104 cells/cm2, respectively. Cells subsequently were incubated in complete growth medium supplemented with: (a) control IgG isotype-matched antibody and DMSO vehicle control; (b) anti-IGF-IR antibody (clone IR-3) and DMSO; (c) control IgG isotype-matched antibody and PD098059; or (d) anti-IGF-IR antibody (clone IR-3) and PD098059. After 4 days, triplicate wells were counted for each treatment. Mean and SD are shown. Data are representative of repeated experiments. When included, 25 µM PD098059 and 400 ng/ml of antibodies were used.