Table 1 PKC C2 and V5 constructs used in this study

Construct PKC isoform, amino acids Primers Forward–Reverse

{alpha}C2E PKC{alpha}, 155–312 F: CGCAGATCTGGACCATGCACACTGAGAAGAGGGGGCGGATT
R: AACGTCGACGCAGGGCCAAGTTTGGCTTT
{delta}C2E PKC{delta}, 1–136 F: CGCAGATCTCCCACCATGGCGCCGTTCCTGCGCATC
R: CGCGAATTCCCTCGTCCTCACTGCGCAT
{varepsilon}C2E PKC{varepsilon}, 1–146 F: GGAAGATCTCGACCATGGTAGTGTTCAAT
R: CGCGTCGACACACGCTCTTCATTGTCTT
{alpha}EV5 PKC{alpha}, 618–672 F: GCGAGATCTGTGTGTGGCAAAGGAGCAGAG
R: CGCGTCGACTCATACTGCACTCTGTAAGAT
ßIEV5 PKCßI, 622–671 F: GCGAGATCTAGAGACAAGAGAGACACCTCC
R: CGCGTCGACCTACACATTAATGACAAACTC
ßIIEV5 PKCßII, 622–673 F: GCGAGATCTTGTGGGCGAAATGCTGAAAAC
R: CGCGTCGACTTAGCTCTTGACTTCGGGTTT
CMSPKCßI PKCßI, 1–671 F: GGAAGATCTGCAAGATGGCTGACCCGGCT
R: CGCGTCGACCTACACATTAATGACAAACTC
CMSPKCßII PKCßII, 1–673 F: GGAAGATCTGCAAGATGGCTGACCCGGCT
R: CGCGTCGACAAGCTCTTGACTTCGGGTTTTA