Fig. 1. PKC C2 and V5 domain constructs. This figure outlines the structure of classical (PKC, PKCßI, and PKCßII) and novel (PKC and PKC) PKC isoforms and highlights in gray the C2 and V5 regions used in this study. SK-N-BE(2) cells were transfected with expression vectors coding for EGFP alone and for PKC, PKC, and PKC C2 domains and PKC, PKCßI, and PKCßII V5 domains fused to EGFP. Cell lysates (60 µg of cellular protein) were subjected to Western blot analysis with polyclonal anti-green fluorescent protein antibody. The positions of molecular weight markers (97, 66, 46, 30, and 21) are indicated (in thousands). Arrowheads indicate the positions of the V5 and C2 domain-EGFP fusion proteins. An unspecific band at approximately Mr 55,000 is also detected with this antibody.