Table 2 Effect of dnPLK1 on cdk1 kinase activity

Cyclin B1 antibody was used to immunoprecipitate cdk1 from control and from dnPLK1 Ad-infected cells prepared as above for cdc25C phosphorylation status. cdk1 activity against Histone H1 substrate was determined.

Cell line Cdk1 kinase activity results:

Phenotype MOI Time (h) % inhibition

HMEC Nonsensitive 500 48 98
U-20S Sensitive 500 24 88
MCF7 Nonsensitive 650 24 85