Table 1 Quantification of abnormal mitoses induced by dnPLK1 in SAOS-2 and U-2OS tumors and normal HMEC cells

Percentages total mitoses were calculated from at least 1000 cells. Percentages of mitoses that were abnormal, unipolar, or abnormal unipolar were determined by examining at least 100 mitoses.

Cell line Treatment Time (h) % total mitoses % abnormal mitoses % monoastral mitoses % abnormal monoastral mitoses

SAOS-2 null Ad 24 4.3 7.7 22 24
SAOS-2 dnPLK Ad 24 43.5 63.0 50.8 41
U-2OS null Ad 24 2.2 9.1 N.D.a N.D.
U-2OS dnPLK Ad 24 43.0 50.8 33.0 43
HMEC null Ad 48 2.8 1.0 12.9 1.0
HMEC dnPLK Ad 48 8.4 2.0 48.0 2.0

a N.D., not done.