Fig. 1. Temperature dependence of the cell death-related properties of ptsp53(Val135). The U-937/ptsp53 clone A2 and the mock-transfected U-937 clone M2 at an initial concentration of 0.2 x 106 cells/ml were grown in suspension culture at 31.5°C, 32.5°C (i.e., temperatures permissive for wild-type p53 activity) and 37°C (i.e., the nonpermissive temperature for wild-type p53-activity) for 4 days. Viability, as judged by trypan blue exclusion, was determined daily. •, ptsp53/A2 31.5°C; {blacksquare}, ptsp53/A2 32.5°C; {diamondsuit}, ptsp53/A2 37°C; {circ}, M2 31.5°C; {square}, M2 32.5°C; {diamond}, M2 37°C. Mean values are from three separate experiments; bars, SE.