Table 2 Mean fluorescence values of mitochondrial mass and {Delta}{psi}m in 15d-PGJ2-treated cells (relative to control cells)

Green and red fluorescence are expressed as a ratio of treated:control. Decreasing red fluorescence indicates a loss of mitochondrial transmembrane potential in correlation with increasing green fluorescence ratio.

10 µM 15d-PGJ2

2.5 µM 15d-PGJ2

6 h 24 h 6 h 24 h

JC-1 red 0.87 ± 0.04a 0.74 ± 0.05a 0.89 ± 0.04a 0.85 ± 0.01a
LNCaP
JC-1 green 2.16 ± 0.44a 2.19 ± 0.35a 1.59 ± 0.26a 1.73 ± 0.20a
Red/green 0.4 ± 0.11a 0.25 ± 0.05a 0.56 ± 0.13a 0.49 ± 0.07a
JC-1 red 0.91 ± 0.03a 0.43 ± 0.03a 1 ± 0.02 0.96 ± 0.05
PC-3
JC-1 green 2.34 ± 0.58a 5.28 ± 0.57a 0.97 ± 0.13 1.28 ± 0.18
Red/green 0.39 ± 0.12a 0.08 ± 0.01a 1.03 ± 0.16 0.75 ± 0.19
JC-1 red 0.89 ± 0.01a 0.56 ± 0.03a 0.98 ± 0.01a 0.83 ± 0.08a
DU145
JC-1 green 4.07 ± 0.62a 5.53 ± 0.52a 1.49 ± 0.47 2.4 ± 0.85a
Red/green 0.22 ± 0.04a 0.1 ± 0.01a 0.66 ± 0.19a 0.35 ± 0.20a

a P <= 0.05 versus control.