Fig. 8. Effect of 15d-PGJ2 treatment on the mitochondrial transmembrane potential of prostate cancer cells. LNCaP, PC-3, and DU145 prostate cancer cells were treated with 15d-PGJ2 for 6 and 24 h. Mitochondrial transmembrane potential was measured using a mitochondria-specific fluorescent probe, JC-1. Red and green fluorescence were measured simultaneously by FACS analysis [excitation at 488 nm; emission 530 nm (green fluorescence) and 585 nm (red fluorescence)]. Events (20,000) were measured, and the number of red or green events is expressed as a percentage of the total (n = 3). P <= 0.05. , red fluorescence; {blacksquare}, green fluorescence.