Fig. 5. Expression of differentiation markers. a, the expression of C/EBP and C/EBPß was studied by Western blotting of LNCaP, PC-3, and DU145 cells treated with 0, 2.5, and 10 µM 15d-PGJ2 for 3, 6, and 24 h. Anti-C/EBP and anti-C/EBPß antibodies (1:500; Santa Cruz) were used for immunodetection. b, a Northern blot of mRNA in LNCaP, DU145, and differentiated 3T3-L1 cells using a probe for aP2, PSA, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH).