Fig. 4. Effects of 15d-PGJ2 treatment on plasma membrane integrity assessed by trypan blue exclusion assay and PI staining. LNCaP, PC-3, and DU145 cells treated with 0, 2.5, and 10 µM 15d-PGJ2 were stained with: a, trypan blue dye; or b, PI and Hoechst 33258 at 2, 4, and 6 h after treatment. The number of blue (a) or red (b) cells per total of 500 was counted and expressed as a percentage of the total (n = 4).