Fig. 2. The effects of 15d-PGJ2 on prostate cancer cell growth analyzed by DNA synthesis. LNCaP, PC-3, and DU145 cells were treated with 0, 2.5, and 10 µM 15d-PGJ2 for 24 and 48 h. Additionally, cells were incubated with 10 µM bromodeoxyuridine solution for 18 h prior to harvesting. The amount of newly synthesized DNA was measured by nonradioactive bromodeoxyuridine ELISA (Roche) and expressed as percentage of controls (n = 4); bars, SD.