Fig. 8. Subcellular distribution of HA-tagged PCTAIRE-1 during cell cycle progression. HeLa cells were transfected with HA-tagged PCTAIRE-1 and synchronized by a double thymidine block. A, cells were fixed in cold methanol before probing with HA or PCTAIRE-1 antibody (left panels) and cyclin antibody (right panels). B, cells in S phase were labeled with BrdUrd (red) and stained with PCTAIRE-1 antibody (green). Bar, 10 µm.