Fig. 7. Comparison of PCTAIRE-1 distribution with the actin and tubulin network. Swiss 3T3 cells were fixed in formalin and permeabilized with Triton X-100 (A and B) or fixed with cold methanol (C and D) before probing with PCTAIRE-1 antibody (A and C), Alexa 594 phalloidin (B), or monoclonal tubulin antibody (D). Cells were observed under a confocal laser scanning microscope. Bar, 20 µm.