Fig. 2. Effect of RA on actin cytoskeletal structure in P388D1 cells. Stably transformed P388D1 cells overexpressing the M6P/IGF2R (C and D) and control P388D1 cells (receptor negative; A and B) were treated with (B and D) or without (A and C) 1 µM RA for 24 h. Cells were fixed, permeabilized, and stained with phalloidin-FITC. Green, actin filament structure; red, cellular cytoplasm was stained with Even-blue.