Fig. 8. No expression of GFAP in the Evi1 clone after RA treatment for 12 days detected by immunofluorescence staining and Northern blot hybridization A, immunofluorescent staining of MAP2 was not detected in wild-type P19 cells 12 days after RA treatment (day 12; Aa) but was detected in the Evi1 clone (D2) under the same conditions (Ab). On the other hand, immunofluorescent staining of GFAP was detected in wild-type P19 cells 12 days after RA treatment (day 12; Ac), but was not detected in the Evi1 clone (D2) under the same conditions (day 12; Ad). B, Northern blot hybridization of GFAP against total RNA from wild-type P19 cells and the Evi1 clone (D2), which were cultured for indicated dates after RA treatment. C, micrographs of wild-type P19 cells at days 4 (Ca) and 12 (Cc) and those of the Evi1 clone (D2) at days 4 (Cb) and 12 (Cd).