Fig. 2. Ability of FGF2 to stimulate the growth of LE-mi-CNR cells. Low-density cultures of G418-selected CNR cells transfected with LE-mi or LE were grown in 22-mm diameter gelatinized wells (4 x 104 cells) with 1 ml of medium containing 1% FCS, 1% MEM/100x vitamins, 10 µg/ml conalbumin, and 5 µg/ml insulin. FGF2 was added 2 days after the day of plating at the concentration indicated in the figure. After 4 days in culture, duplicate wells were trypsinized, and cells were counted. •, LE-mi CNR cells; {blacksquare}, LE CNR cells.