Table 2 Effect of aphidicolin treatment on the subcellular distribution profile of p94fer
|CHO cells were transiently transfected with a p94fer expression vector, left untreated, or exposed to aphidicolin for the last 20 h of transfection. Some samples were treated with aphidicolin for the last 17 h of transfection; the drug was then washed away, and the cells were replenished with fresh medium for a further 3 h. Cells were fixed, and the subcellular distribution profiles of p94fer were determined using indirect immunocytochemistry and confocal microscope analysis.
|Aphidicolin + 3 h
a Cytoplasmic, cells that harbored 040% of the p94fer molecules in the cytoplasm; Equal, cells that harbored 4060% of the p94fer molecules in the cytoplasm or in the nucleus; Nuclear, cells that harbored 60100% of the p94fer molecules in the cell nucleus.