Fig. 2. The half-life of the p53 protein in revertants is increased 4-fold over that seen in the parental HeLa cells. A, a representative Western blot analysis of cellular lysates from HeLa, HA, and HF at various intervals after treatment with the inhibitor of protein synthesis CHX and probed with anti-p53 antibody (DO-1). B, quantitative analysis of Western blots by laser densitometry. The average densitometric values from three independent experiments were plotted as a percentage of p53 remaining as a function of time after CHX treatment. The p53 half-life for each cell line was calculated using the regression equation. {circ}, HeLa, {square}, HA, {diamond}, HF.